Heterogeneousin situ immunophenotyping of follicular dendritic reticulum cells in malignant lymphomas of B-cell origin

Cancer ◽  
1987 ◽  
Vol 60 (12) ◽  
pp. 2919-2926 ◽  
Author(s):  
Antonino Carbone ◽  
Alessandro Poletti ◽  
Riccardo Manconi ◽  
Annunziata Gloghini ◽  
Rachele Volpe
Keyword(s):  
B Cell ◽  
Malignant Lymphomas ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Follicular Dendritic

scholarly journals The immunohistology of follicle lysis in lymph node biopsies from homosexual men

Blood ◽  
1985 ◽  
Vol 66 (5) ◽  
pp. 1092-1097
Author(s):  
GS Wood ◽  
CF Garcia ◽  
RF Dorfman ◽  
RA Warnke
Keyword(s):  
Lymph Node ◽  
B Cell ◽  
B Cell Lymphoma ◽  
Cell Phenotype ◽  
Homosexual Men ◽  
Retroviral Infection ◽  
T Lymphotropic Virus ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Immunodeficiency Syndrome

Follicle lysis is a characteristic alteration of B cell follicles described recently in lymph node biopsies from homosexual men. It consists of disruption of germinal centers by aggregates of small mature lymphocytes variably associated with erythrocyte extravasation. We studied the immunohistology of follicle lysis identified in lymph node biopsies from 11 homosexual men. The results indicate that follicle lysis has two principal immunohistologic features: (1) intrafollicular aggregates of small lymphocytes predominantly of polytypic mantle B cell phenotype (T015+/Leu-8+/mu+/delta+/k+ or lambda+), and (2) disruption of the normal, unified follicular meshwork of R4/23+ dendritic reticulum cells by these B cell aggregates. These structural alterations may affect the functional integrity of the germinal center as it pertains to the abnormal B cell effector function and the increased prevalence of B cell lymphoma recently documented in the acquired immunodeficiency syndrome and related disorders. Because dendritic reticulum cells weakly express the Leu-3 (T4) antigen, which is known to be an essential component of the receptor for human T- lymphotropic virus type III/lymphadenopathy-associated virus (HTLV- III/LAV) retrovirus infection, it is possible that retroviral infection of dendritic reticulum cells may play a role in the pathogenesis of follicle lysis.

scholarly journals Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood

Blood ◽  
1983 ◽  
Vol 62 (3) ◽  
pp. 585-590 ◽  
Author(s):  
MR Parwaresch ◽  
HJ Radzun ◽  
ML Hansmann ◽  
KP Peters
Keyword(s):  
Monoclonal Antibody ◽  
Dendritic Cells ◽  
Specific Antigen ◽  
Electron Microscopic Level ◽  
Mononuclear Leukocytes ◽  
Immunoperoxidase Method ◽  
Follicular Dendritic Cells ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Follicular Dendritic

Ki-M4, a new IgG3 monoclonal antibody, selectively recognizes dendritic reticulum cells (DRC; follicular dendritic cells) in all human lymphatic organs, as tested by the immunoperoxidase method on the light and electron microscopic level. This antibody was raised against separated lysosomes of the 12–0-tetradecanoyl phorbol-13-acetate (TPA) stimulated permanent cell line, U-937, derived from a human histiocytic lymphoma. No cross-reactivity was encountered in epithelial and mesenchymal cells, including macrophages and other cells detectable in bronchial and peritoneal lavages. In the nonadherent fraction of the mononuclear blood cells collected from the interphase of a Ficoll- Urografin gradient (density = 1.077 g/ml), 0.1 per million of the cells hitherto not classified as monocytes or lymphocytes showed a strong reaction. All other separated blood cell types were devoid of any reactivity. The observation that DRC share a highly restricted, and thus specific, antigen with a small but distinct subpopulation of mononuclear leukocytes implies their blood derivation.

Follicular Dendritic Reticulum Cells and Lymphomas of Follicular Origin

1987 ◽  
Vol 88 (2) ◽  
pp. 249-250 ◽  
Author(s):  
Riccardo Manconi ◽  
Alessandro Poletti ◽  
Sandro Sulfaro ◽  
Rachele Volpe ◽  
Antonino Carbone
Keyword(s):  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Follicular Dendritic

scholarly journals Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood

Blood ◽  
1983 ◽  
Vol 62 (3) ◽  
pp. 585-590 ◽  
Author(s):  
MR Parwaresch ◽  
HJ Radzun ◽  
ML Hansmann ◽  
KP Peters
Keyword(s):  
Monoclonal Antibody ◽  
Dendritic Cells ◽  
Specific Antigen ◽  
Electron Microscopic Level ◽  
Mononuclear Leukocytes ◽  
Immunoperoxidase Method ◽  
Follicular Dendritic Cells ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Follicular Dendritic

Abstract Ki-M4, a new IgG3 monoclonal antibody, selectively recognizes dendritic reticulum cells (DRC; follicular dendritic cells) in all human lymphatic organs, as tested by the immunoperoxidase method on the light and electron microscopic level. This antibody was raised against separated lysosomes of the 12–0-tetradecanoyl phorbol-13-acetate (TPA) stimulated permanent cell line, U-937, derived from a human histiocytic lymphoma. No cross-reactivity was encountered in epithelial and mesenchymal cells, including macrophages and other cells detectable in bronchial and peritoneal lavages. In the nonadherent fraction of the mononuclear blood cells collected from the interphase of a Ficoll- Urografin gradient (density = 1.077 g/ml), 0.1 per million of the cells hitherto not classified as monocytes or lymphocytes showed a strong reaction. All other separated blood cell types were devoid of any reactivity. The observation that DRC share a highly restricted, and thus specific, antigen with a small but distinct subpopulation of mononuclear leukocytes implies their blood derivation.

scholarly journals The immunohistology of follicle lysis in lymph node biopsies from homosexual men

Blood ◽  
1985 ◽  
Vol 66 (5) ◽  
pp. 1092-1097 ◽  
Author(s):  
GS Wood ◽  
CF Garcia ◽  
RF Dorfman ◽  
RA Warnke
Keyword(s):  
Lymph Node ◽  
B Cell ◽  
B Cell Lymphoma ◽  
Cell Phenotype ◽  
Homosexual Men ◽  
Retroviral Infection ◽  
T Lymphotropic Virus ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Immunodeficiency Syndrome

Abstract Follicle lysis is a characteristic alteration of B cell follicles described recently in lymph node biopsies from homosexual men. It consists of disruption of germinal centers by aggregates of small mature lymphocytes variably associated with erythrocyte extravasation. We studied the immunohistology of follicle lysis identified in lymph node biopsies from 11 homosexual men. The results indicate that follicle lysis has two principal immunohistologic features: (1) intrafollicular aggregates of small lymphocytes predominantly of polytypic mantle B cell phenotype (T015+/Leu-8+/mu+/delta+/k+ or lambda+), and (2) disruption of the normal, unified follicular meshwork of R4/23+ dendritic reticulum cells by these B cell aggregates. These structural alterations may affect the functional integrity of the germinal center as it pertains to the abnormal B cell effector function and the increased prevalence of B cell lymphoma recently documented in the acquired immunodeficiency syndrome and related disorders. Because dendritic reticulum cells weakly express the Leu-3 (T4) antigen, which is known to be an essential component of the receptor for human T- lymphotropic virus type III/lymphadenopathy-associated virus (HTLV- III/LAV) retrovirus infection, it is possible that retroviral infection of dendritic reticulum cells may play a role in the pathogenesis of follicle lysis.

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